A color change from purple to yellow indicates surviving spores and a positive result. Then, the BI is incubated for a visual color change readout at 48 hours. After the steam sterilization process, the BI is activated to join the growth media with the processed spore strip. A great example of reevaluating and improving BI testing in a practice is moving away from 24- to 48-hour spore testing results and mail-in testing, to receiving test results in less than one hour, such as with the 3M Attest biological monitoring system. When processed, the chemical process indicator changes from rose to brown/black. The spore strip and media ampoule are contained in a plastic vial topped with a brown color-coded cap which includes a chemical process indicator on the label. This self-contained biological indicator (BI) consists of a Geobacillus stearothermophilus spore strip and a sealed glass ampoule with growth medium and a bromocresol purple pH indicator system. The ISO 11138-1:2017 and ISO 11138-3:2017 compliant 3M™ Attest™ Steam Biological Indicator, 1262/1262P, monitors the effectiveness of the steam sterilization process for 250☏/121☌ gravity displacement and 270☏/132☌ vacuum-assisted steam sterilization cycles and provides a final result in 48 hours. A colour change to yellow indicates surviving spores and a positive result. The 3M™ Attest™ Steam Biological Indicator, 1262/1262P, Brown Cap, monitors 250☏/121☌ gravity displacement and 270☏/132☌ vacuum-assisted saturated steam sterilization cycles and provides a final result in 48 hours. The BI is incubated for 48 hours for a visual change readout.
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